A hamstrings autograft is when the hamstrings tendon (semitendinosus) is surgically removed for use as a graft in the same person.
The semitendinosus hamstring tendon is harvested on the medial side of the knee, often together with the gracilis tendon.
The prepared graft ready for the reconstruction procedure.
Hamstrings graft harvest
An incision is made on the inner side of the knee over the pes anserinus (where the tendons join like a goose foot), and a tendon-stripper instrument is pushed up to strip out part of the tendons, which are then folded and sutured to make a stronger graft.
ACL Reconstruction Basics: Quadruple (4-Strand) Hamstring Autograft Harvest Frank RM, Hamamoto JT, Bernardoni E, Cvetanovich G, Bach BR,Jr., Verma NN and Bush-Joseph CA. Arthrosc Tech. 2017 Aug; 6(4): e1309–e1313.