Human platelet lysate is a blood extract that causes platelets to break down, releasing a large quantity of growth factors necessary for growing cells in the laboratory.
Preparation of human platelet lysate
Blood is taken from healthy human donors and the platelet layer separated off by centrifuge. The platelet concentrate is pooled from several donors, and subjected to freezing and thawing to break down (lyse) the platelet wall, and the released the 'lysate' - full of growth factors - is used in laboratory processes.
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Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells.. Hemeda H, Giebel B and Wagner W. Cytotherapy. 2014 Feb;16(2):170-80. doi: 10.1016/j.jcyt.2013.11.004.